Any of several techniques for separating macromolecules based on their migration in a gel or other medium subjected to a strong electric field. ( Figure 3-41)
A technique by which DNA fragments are placed in a gel and charged with electricity. The process separates the fragments by size, as part of the process of creating a genetic profile.
A process by which enzymes are separated based on differences in electrical charge. Observed differences between or within species indicate mutation and genetic divergence over long periods of time, thus elucidating the inter-relationships of organisms.
Method using an electrical field which leads to the separation of proteins or DNA fragments based on their size. Smaller proteins or DNA fragments move faster; larger ones slower. Samples are normally placed in the electrical field loaded in a gel-like substance, called agar or agarose.
a laboratory procedure which separates molecules according to their electric charge.
separation technique based on movement in an electric field
a technique that involves the resolution of macromolecules such as proteins or nucleic acids by migration through a gel under the influence of an electrical field.
a method used to separate hemoglobin in the identification and diagnosis of hemoglobinopathies[close window
migration of charged molecules in an electric field through a medium such as polyacrylamide gel; usually performed for analytical purposes — but may be used to partially purify molecules for other analytical methods such as mass spectrometry, PCR, cloning, DNA sequencing, or immunoblotting.
The separation of ions (eg. proteins) in an electric field. For macromolecules, this is usually performed in a water-swellable matrix like polyacrylamide or agarose which performs sieving functions and reduces convection.
The use of an external electric field to separate large biomolecules on the basis of their charge by running them through acrylamide or agarose gels.
a technique used by biochemists to separate and identify large biological molecules by using electric current
A technique that separates dissolved or colloidal particles subjected to an electrical field according to their mobilities. Electrophoretic mobility depends on the size, three-dimensional geometry, and electrical charge of the particle.
A lab technique for sorting organic molecules into various weight groups by exposing them to an electric field.
A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field Escherichia coli A bacterium that is a common found in the intestinal tract of warm blooded animals.
is the motion of particles (of atomic or larger size) under the influence of an electric field. This motion implies that the particles bear an electric charge.
A laboratory test in which a patientâ€(tm)s serum molecules are subjected to separation according to their size and electrical charge. For myeloma patients, electrophoresis of the blood allows both the calculation of the amount of myeloma protein (m-protein) as well as the identification of the specific M-spike characteristic for each patient. Electrophoresis is used as a tool both for diagnosis and for monitoring.
A method of separating electrically charged molecules (e.g., ions of DNA or protein)) in an electric field by passage through an agarose or acrylamide gel (or other) that separates on the basis of electric charge and mass of the molecule.
Technique of applying an electrical charge to primary plasma proteins and observing their migration on paper.
A technique that separates mixtures of charged particles by differences in their rate of migration in an applied electric field.
The movement of charged particles through a fluid following the application of an electric field.
An experimental technique to fractionate (separate) charged molecules that relies on differential migration velocities through an obstructive medium (gel), under the influence of an applied electric field.
The motion of colloidal species caused by an imposed electric field.
a process whereby DNA fragments are sorted according to length ( i.e., number of short tandem repeats). In general, the process involves: 1) Adding DNA to one end of a piece of gelatinous material which contains tiny holes that allows the material to function as a molecular sieve; 2) applying an electric current to the material, causing the DNA fragments to move; and 3) determining the size of the DNA fragments by comparing the distance each fragment moved to the distances moved by the fragments of known size. Since it is easier for smaller fragments to move through the material, the smaller fragments move farther than the larger fragments. As a result, at the end of electrophoresis the DNA fragments are sorted by size. Electrophoresis is the fourth of five stages in the PCR/STR analysis process.
Laboratory test used to measure the levels of various proteins in the blood or urine. Uses an electrical current to sort proteins by their molecular size.
A technique for separating a mixture of different molecules by causing the molecules to move through an electric field.
A technique used to separate molecules, normally according to size.
The technique of separating different sized DNA fragments by placing them in a gel and subjecting it to a strong electromagnetic field. Smaller fragments will generally migrate further through the gel in a given time than larger ones.
A technique for arranging biological particles across a gel medium by exposing them to an electric field.
the motion of charged particles in a colloid under the influence of an electric field; particles with a positive charge go to the cathode and negative to the anode
an analytical technique used to separate DNA fragments by size
an open ended simulation which cuts any DNA sequence with any combination of restriction enzymes runs realistic gels and Southern blots of the resulting DNA fragments and converts DNA fragments to mapping fragments for restriction mapping
The separation and identification of molecules based on their movement through an electrically charged field.
The movement of particles between electrodes through a liquid or gel by the contact of an electrical current. It is known as a safe and effective means of absorption of bee venom.
The process whereby an electric charge is used to separate molecules in a solution or gel according to electrical charge and size. It is routinely used to separate fragments of DNA.
The process by which charged particles move, typically through a gel, in response to an electric field.
A technique used to separate molecules such as DNA or proteins using an electric current. The mixture of molecules is added to one end of a gel-like medium. When a current is applied to it, the molecules will travel through the medium to the other end at different speeds depending on the charge and size of the molecule. Once the molecules are separated, the gel can be used in a blot (Southern, Northern and Western).
A technique for separating and analyzing DNA, RNA, or proteins based on physical attributes in an electric field through a gel.
The process by which charged molecules are separated by their different mobilities in an electric field.
Français] A lab procedure in which DNA fragments are separated in a gel that is placed in an electric field. This is one of the steps in DNA fingerprinting.
Electrophoresis is the movement of charged particles in an electric field. This process has been developed as an analytical tool to detect genetic variation revealed by charge differences on proteins or molecular weight in DNA. Data obtained by electrophoresis can provide insight into levels of genetic variability within populations and the extent of genetic differentiation between them.
A process that separates pieces of DNA by length as a consequence of differential electric charge.
using different electrical characteristics of amino acid sequences from proteins from tissues (of fish) to determine genetic make up.
A technique for separating the components of a mixture of charged molecules (proteins, DNAs, or RNAs) in an electric field within a gel or other support.
A nonemissive flat panel technology based on the movement of charged particles (of one color) in a colloidal suspension (of a second color) under the influence of an electric field. The application of the electric field changes the absorption or transmission of light through the solution.
A process whereby charged molecules (such as DNA and enzymes) are separated in an electric field.
A technique that uses an applied voltage to separate different molecules. It works particularly well for large molecules like proteins or DNA. DNA molecules are separated on the basis of their size. Depending on exactly how the electrophoresis is done, protein molecules can be separated on the basis of size or of a combination of size, shape and charge.
Movement of suspended particles through a fluid or gel under the influence of an electrical charge.
The method of separating and sorting proteins by drawing them through a fine gel using an electrical current, allowing them to be identified.
The separation in an electrical field of the macromolecular constituents in a mixture (proteins, nucleic acids).
In genetics, the technique for separating materials via the movement of charged particles suspended in a gel under the influence of an applied electric field.
technique for separating proteins such as enzymes, based on their different mobilities in an electric field. It is used to identify species that may look similar and to distinguish sub-populations or stocks.
A technique used to evaluate genetic components based on their migration in an electric field. The technique separates alleles into different regions so they can be differentiated. Electrophoresis is commonly used to evaluate red cell enzymes, serum proteins and DNA
An established method for separation of biomolecules, such as DNA fragments or proteins, from a mixture prior to their further analysis and identification (e.g. by fluorescence detection or mass spectrometry). The analyte mixture is applied onto a polymer medium (gel) at a defined position. An electrical current drives the charged biomolecules through the gel. These are separated based on their different retardation by the gel, depending on their charge and size.
A method of separating complex protein molecules suspended in a gel by running an electric current through the gel.
The use of electrical fields to separate charged biomolecules such as DNA, RNA, and proteins. DNA and RNA carry a net negative charge because of the numerous phosphate groups in their structure. In the process of gel electrophoresis, these biomolecules are put into wells of a solid matrix typically made of an inert substance such as agarose. When this gel is placed into a bath and an electrical charge applied across the gel, the biomolecules migrate and separate according to size in proportion to the amount of charge they carry. The biomolecules can be stained for viewing and isolated and purified from the gels for further analysis. Electrophoresis can be used to isolate pure biomolecules from a mixture or to analyse biomolecules (such as for DNA sequencing).
The separation of ions in an electric field. For macromolecules, this is usually done in a water-swellable matrix like polyacrylamide or agarose which perform sieving functions and reduce convection.
the movement of molecules (such as proteins and nucleic acids) through a gel due to electrical poles established by electrodes in contact with the medium. The process separates molecules into bands according to size
Separation of components of a mixture by their differing migration in an applied electric field.
A procedure by means of which the members of a heterogenous population of charged particles can be separated by virtue of their dissimilar migration characteristics in an electric field. ( 16)
A method of separation of molecules in a mixture; an electric current is passed through the mixture which has been loaded on a support medium, usually a gel or sometimes paper, the molecules move through the support - the extent of each molecule's movement is related to its size and its charge.
A laboratory procedure in which DNA pieces are size-sorted by traveling through microscopic openings in an agarose gel in response to an electrical field.
A method of separating large molecules, such as DNA fragments or proteins, from a mixture of similar molecules.
slow movement of colloidal particles in an electric field; a process used to separate and identify compounds.
In electrophoresis, DNA to be sequenced is placed at one end of a gel—a slab of a gelatin-like substance. (IOCeleraGenome) Eletroforese... foi clivado com a enzima de restrição EcoRI, fracionado por eletroforese em gel de agarose e analisado em ... (POUniverRS)
Method used to separate protein or nucleic acid molecules in an electric field extending across a physical medium such as agarose gel or polyacrylamide.
The movement of molecules or other very small particles through a fluid under the action of an external electric field. Positively charged particles (metallic oxides, basic dyestuffs) migrate to the cathode, and negatively charged particles (metals, sulfur, metallic sulfides, acid dyestuffs) migrate to' the anode.
a technique that separates DNA fragments on the basis or their size by running them through a viscous material. The fragments move through the material due to the application of an electric current which attracts the negatively charged DNA to the positive terminal, and the fragments are separated due to the fact that the smaller fragments will move through the matrix faster than the larger fragments and will thus arrive sooner at the positive terminal.
refers to the process of using an electric field to move molecules in a solution. In the SDS PAGE experiment, these molecules are protein molecules in solution with SDS. For the molecules to be influenced by the electric field, they must have a net charge.
is the process in which molecules (such as proteins, DNA, or RNA fragments) can be separated according to size and electrical charge by applying an electric current to them. The current forces the molecules through pores in a thin layer of gel, a firm jelly-like substance. The gel can be made so that its pores are just the right dimensions for separating molecules within a specific range of sizes and shapes. Smaller fragments usually travel further than large ones. The process is sometimes called gel electrophoresis.
A technique for separation of ions by rate and direction of migration in an electric field.
test in which blood or urine samples are checked for M proteins
A technique by which DNA fragments are placed in a gel and separated by size in response to an electrical field.
Electrophoresis is the technique of separating electrically charged particles, particularly proteins, in a solution by passing an electric current through the solution. The rate of movement of the different components depends upon their charge, so that they gradually separate into bands. Electrophoresis is widely used in the analysis of body chemicals, such as the analysis of the different proteins in blood serum.
The technique of separating charged molecules in a matrix to which is applied an electrical field
a method for separating proteins or nucleic acids on the basis of their electric charge and size.
The method by which biological molecules are separated according to certain characteristics. In DNA parentage testing the negatively charged DNA fragments are cut and separated by size in a porous material such as agarose, by application of an electric field.
The primary method used to separate the mixture of nucleotide or peptide fragments generated from DNA or protein cleavage experiments. The apperatus consists of a plate, coated with either agarose or acrylamide gels, which is placed in an electric field. As the solvent is allowed to infuse up the length of the plate, the components of the mixture are separated by size. In addition, the electrical charge along the side of the plate forces migration of the DNA or protein fragments according to the net charge of the residues.
The drawing of a mixture of proteins through a fine gel by an electric current. This separates the proteins so that they can be identified.
separation of molecules (proteins or nucleic acids) in an electric field as a function of their molecular weight and/or their electric charge.
a technique in which molecules are separated by their velocity in an electric field
The movement and separation of charged molecules in solution in an electrical field.
Technique for analyzing and separating molecules based on the movement of charged particles in an electric field.
Movement of charged particles in an electrical field, a technique used to separate materials in a substance by placing a sample in a buffer solution through which an electrical current then is passed. Extent of migration and separation of components depends on molecular weight and electrical charge of the particles. This method often is used to determine the proportions of various proteins in serum.
Fractionating of protein in an electric field. It enables both the calculation of the myeloma protein amount, as well as evidence of the M-apex (monoclonal immunoglobulin). Electrophoresis is used in diagnosis, as well as for the monitoring of the therapy.