IHC. A laboratory test that uses antibodies to detect particular proteins or receptors in cells or tissue samples. IHC tests may be used to help determine whether breast cancer cells have hormone receptors or too much of the HER2/neu protein. Results from IHC can help determine breast cancer treatment options.
Technique that uses antibodies to identify and mark antigens expressed by cells in tissues.
The use of antibodies or antisera as histological tools for identifying patterns of antigen distribution within a tissue or an organism. An antibody (or mixture of antibodies) that binds to a specific protein or other antigen is tagged with a fluorescent chemical or an enzyme that can convert a substrate to a visible dye. The tagged antibody is incubated with the tissue and after washing unbound antibody away the bound antibody distribution is revealed by fluorescence microscopy or incubation with a chromogenic substrate. It is assumed that antibody distribution reflects antigen distribution.
A technique that uses antibodies to identify and measure specific protein molecules in tissue sections using a microscope.
an assay that shows specific antigens in tissues by the use of markers that are either fluorescent dyes or enzymes (such as horseradish peroxidase)
A technique to detect the presence of an antigen in histologic tissue sections by use of an enzyme-coupled antibody that is specific for the antigen. The enzyme converts a colorless substrate to a colored insoluble substance that precipitates at the site where the antibody and thus the antigen are localized. The position of the colored precipitate, and there for the antigen, in the tissue section is observed by conventional microscopy Immunohistochemestry is a routine technique in diagnostic pathology and various fields of research (1).
A form of tissue staining utilizing the specific binding properties of antibodies.
a test done on a tissue sample of a tumor to determine EGFR status (usually for colon cancer).
Used primarily by pathologists and oncologists assisting in the diagnosis of cancer and the determination of different treatment options. IHC staining is used to test for the presence or over expression of the proteins involved in cancer.
of or relating to the application of tissue chemistry and immune reaction methods to analysis of living cells and tissues
the testing of samples of tissue from an animal for specific proteins by attaching specific antibodies to them, and then marking these antibodies with enzymes to which they are connected.
Chemical techniques used to indentify the deposit of particular chemicals or proteins in tissue. Common used to locate prion protein in brain tissue from SE using an anti-PrP antibody.
Chemical methods used to locate deposits of particular chemicals or proteins in tissues. Often used to identify the prion protein in brain tissue.
A technique that utilizes specific antibodies for staining tissues on microscopic glass slides. This enables the pathologist to correctly identify many different types of tumor cells which leads to a more accurate final diagnosis.
A technique used to demonstrate the presence of an antigen in various tissue lesions (often fixed in formalin).
The testing of sections of tissue for specific proteins by attatching them with specific antibodies, and then looking for the antibodies through the enzymes to which they were connected.
Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues. Immunohistochemical staining is widely used in the diagnosis of cancer. Specific molecular markers are characteristic of particular cancer types.