John Mackey (born September 24, 1941, New York, New York) is a former American Football tight end who played for the Baltimore Colts (1963-1971) and the San Diego Chargers (1972). He starred collegiately at Syracuse University, where his nephew Jerry Mackey is currently a linebacker. In 1963, Mackey joined Colts and by 1966 had revolutionized the position of tight end in the NFL.

John Mackey is Chairman and CEO of Whole Foods Market (which he co-founded in 1980), a $5.7 billion sales Fortune 500 company, and a "Fortune 100 Best Companies To Work For" every year since 1998. Whole Foods is one of the top 12 supermarket companies in America and the world's largest natural foods retail chain. Mackey was named the Ernst and Young Entrepreneur Of The Year in 2003.

John Mackey is an American mathematician. He received his Ph.D. in mathematics from the University of Hawaii at Manoa in 1994 and is currently a lecturer and assistant department head at Carnegie Mellon University.