A cellulose or nylon membrane to which RNA molecules have been attached by capillary action. The transferred RNA is hybridized to single-stranded DNA probes. northern blot technique is often used to measure expression (transcription) of a gene for which a specific cDNA is available for use as a probe. See Southern blot, western blot.
A technique used to identify and locate mRNA sequences that are complementary to a piece of DNA called a probe.
An electroblotting method in which RNA is transferred to a filter and detected by hybridization to 32P-labelled RNA or DNA.
a Southern blot with RNA instead of DNA
RNA blot taken from an electrophoresis gel.
(synonym: northern blotting analysis) The separation of sequences or fragments of RNA, partially digested by endonucleases, on an electrophoretic gel
The transfer of size-separated RNA fragments to a synthetic membrane for further studies.
A gel-based laboratory procedure that locates mRNA sequences on a gel that are complementary to a piece of DNA used as a probe. Source : Human Genome Project Information
A gel-based laboratory procedure that locates mRNA sequences on a gel that are complementary to a piece of DNA used as a probe. See also: DNA, library
A technique used to separate and transfer mRNA from a gel to a filter in order to identify and locate mRNA sequences that are complementary to and hybridize with a labeled DNA probe.
RNA from a sample is spatially separated and distributed by mass on a gel. Radioactively labelled DNA or RNA strands with sequence complementary to the RNA segments from the sample are used to locate the position of those RNA segments.
(NB), uses techniques similar to the Southern blot described above. Messenger-RNA from the specimen is separated by electrophoresis and blotted to a specifically modified paper support to result in covalent fixing of the mRNA in the electrophoretic positions. Radiolabeled single-stranded DNA fragments complementary to the specific mRNA being sought are then hybridized to the bound mRNA. If the specific mRNA is present, the radioactivity is detected by autoradiography. The derivation of this technique from the Southern blot used for the DNA detection has led to the common usage of the term "Northen blot" for the detection of specific mRNA.
Transfer of electrophoretically separated RNA molecules from a gel onto an absorbent sheet, which is then immersed in a labeled probe that will hybridize to an RNA of interest to reveal its presence. See Southern blotting.
technique for analyzing RNAs, in particular messenger RNA, by electrophoresis followed by transfer and hybridization with a labeled nucleotide marker on a filter.
The method for separating RNA fragments via electrophoresis and then immobilizing them on a membrane support.
An electrophoresis-based technique which is used to find mRNA sequences that are complementary to a piece of DNA called a probe.
An RNA blot. This term originated as lab jargon; not a generally accepted term. ( 19)
The northern blot is a technique used in molecular biology research to study gene expression. It takes its name from the similarity of the procedure to the Southern blot procedure, named for biologist Edwin Southern, used to study DNA, with the key difference that RNA, rather than DNA, is the substance being analyzed by electrophoresis and detection with a hybridization probe.