In nuclear transfer the DNA is removed from an unfertilized egg and the nucleus of a specially prepared body cell is introduced and the combination or "couplet" is triggered either by an electrical pulse, or the introduction of a chemical, to fuse them together and begin the process of development. Much remains to be learned about what happens in this process, and most attempts fail at the start.
moving an part or all of an organism's genetic information into an unfertilized egg (whose nucleus had previously been removed); can be used for cloning or to produce transgenic animals (if the genes put into the egg have been recombined with genes from others species).
transfer of a diploid cell nucleus from a donor cell into another cell that has had its original nucleus removed (enucleated cell).
A technology by which animals are created by cloning a single diploid somatic cell. It involves taking a single diploid cell from a culture of cells, and inserting it into an enucleated ovum, i.e., an ovum from which the haploid nucleus has been removed. The resultant diploid ovum develops into an embryo that is placed in a recipient female, which gives birth to the cloned animal in the normal manner. Note that the term is somewhat of a misnomer, since it is a whole cell that is transferred, not just the nucleus.
Replacing the nucleus of one cell with the nucleus of another cell.
A procedure in which a nucleus from a donor cell is transferred into an enucleated egg or zygote (an egg or zygote from which the nucleus/ pronuclei have been removed). The donor nucleus can come from a Germ cell or a somatic cell.
The generation of a new animal nearly identical to another one by injection of the nucleus from a cell of the donor animal into an enucleated oocyte of the recipient.
removal of nucleus from one cell (the donor) and insertion into an oocyte (the recipient) from which the nuclear material has been removed.
A laboratory procedure in which a somatic donor cell is fused with an egg cell which has had its nucleus (containing its chromosomal DNA) removed. This procedure results in the transfer of the donor cell nucleus to the egg cell.
Transferring a somatic cell nucleus into an oocyte that has had its nucleus removed.
In his still-experimental technique, Dr. Jamie Grifo of New York University Medical Center takes the nucleus of an immature egg (i.e., one that hasn't gone through cell division) from the mother-to-be and places it in the enucleated (nucleus-free) egg donated by a young woman. Grifo's hope is that the technique, when perfected, will help older women whose eggs are less than ideal have babies using a younger person's eggs.
the process of removing the nucleus from one cell and implanting it in another
technology where a nucleus from one cell is transferred into an enucleated cell (most of the time an embryo).
Also known as “somatic cell nuclear transfer (SCNT)â€, this is when cells are copied so that their genetic material, or DNA, matches exactly. To carry out the process, scientists remove the nucleus (containing the DNA) from an unfertilized egg and replace it with the nucleus (containing the DNA) from the cell of a patient who has a disease. The egg, with the new genetic material, converts to a blastocyst which contains stem cells that match the patient and has the disease. This offers scientists a unique way to learn about diseases and test new therapies on human cells. By testing therapies on human cells before testing them on living people, scientists can make therapies more safe and effective. This could also reduce risk of clinical trials and the time it takes to get therapies to patients. Scientists hope to use this technique to better understand diseases by developing stem cell lines that have a specific disease. These are known as disease specific or patient specific stem cells. Stem cells derived from SCNT may eventually be useful as therapeutics, as they will not be rejected by the individual from which the nucleus was obtained.
The process by which all of the chromosomal DNA is removed from an egg cell and replaced with the nucleus containing all of the chromosomal DNA from a donor somatic or non-reproductive cell. Fusion between the egg cell and the donor somatic nucleus results in a new cell that gains a complete set of chromosomes derived entirely from the donor nucleus. See also: DNA, Somatic Cell.
Transferring the nucleus with its chromosomal DNA from one (donor) cell to another (recipient) cell. In cloning, the recipient is a human egg cell and the donor cell can be any one of a number of different adult tissue cells.
A laboratory procedure in which a cell's nucleus is removed and placed into an oocyte with its own nucleus removed so the genetic information from the donor nucleus controls the resulting cell.
