The two complementary single-stranded ends of a DNA duplex. For example, resulting from digestion with a Class II restriction endonuclease.
Single strands of DNA that protrude from opposite ends of a duplex. Usually, generated by staggered cuts of a double-stranded DNA by a restriction enzyme.
On a piece of two-stranded DNA, short, complementary, one-stranded regions produced by the action of a restriction endonuclease. Sticky ends allow the joining of segments of DNA from different sources.
When certain restriction enzymes cut DNA, they don't cut through both strands of DNA at the same exact point. We refer to the end they leave as a "sticky end." One strand is left with unpaired bases, which can later pair up with another sticky end created by the same enzyme. Because different restriction enzymes recognize different sequences of DNA, they leave different unpaired bases, which is why a BamHI sticky end can't base pair with a HindIII sticky end. (Other restriction enzymes leave blunt ends (not sticky)--these are not as commonly used by geneticists because they can only "cut" the DNA, and not "paste" it.)
The staggered ends of complementary sequences of DNA which result from cleavage by restriction enzymes. [IUPAC Biotech
After cutting with restriction enzymes, the ends of DNA fragments are "sticky". They can easily be joined with other fragments that were cut with the same enzyme and so have complementary sticky ends.
Term applied to DNA sequences cut with restriction enzymes where the cuts will bond with each other or with another sequence cut with the same enzyme.
On the ends of a restriction fragment of double stranded DNA, there may be a short, single stranded piece of DNA . This is known as a sticky end, since it may be used to join two or more different restriction fragments together where they have complimentary sticky ends. This is very useful in the formation of recombinant DNA molecules.
are complementary single strands of DNA that protrude from opposite ends of a duplex or from ends of different duplex molecules; can be generated by staggered cuts in duplex DNA.
