Cloning vector used to carry large segments of DNA into and out of cells; derived from bacteriophage lambda.
Cloning vector that typically contains insert sizes of 60-120kb. These vectors are hybrids of lambda phages and plasmids and can be propagated as plasmids or packaged like phage. The name comes from the fact that these vectors retain the phage cos sites that are used for lambda head stuffing. These are generally maintained in multiple copies in E. coli. Read more about cosmids references: Evans GA et al. High efficiency vectors for cosmid microcloning and genomic analysis. Gene 1989; 79(1):9-20. Coulsan A et al. The physical map of the Caenorhabditis elegans genome. Methods Cell Biol 1995; 48:533-50.
A type of vector used to clone large DNA fragments. ( Figure 7-16)
A phage lambda-based vector used for cloning large DNA fragments (45 kb) into E. coli cells.
a bacterial plasmid with the cos sequence from the lambda phage
An artificial hybrid vector into which large (~40kbp) DNA fragments can be inserted; this makes it useful in genomic library preparation. Cosmids replicate as plasmids in the host cell, but are inserted into the bacterium from lambda phage infectious particles, since transformation would be extremely inefficient with plasmids of this size. The cosmids carry cos sequences from lambda phage so that they can be packaged into phage heads (in vitro ), and circularise once in the cell.
A large plasmid used as a vector for cloning medium-length (about 40-50 kb) DNA sequences. See also Kilobase (kb).
A vector that incorporates components both of plasmids and the COS region of bacteriophage l , the combination of which enable it to be replicated in a bacterium (e.g. E. coli), and packaged in bacteriophage l coats. May carry relatively large DNA fragments (up to 40 Kilobases).
A cloning vector that, like a plasmid, can replicate autonomously and be packaged into phage.
A cloning vector consisting of the phage lambda cos site inserted into a plasmid. Such vectors can be packaged into lambda phage or maintained as plasmids. Cosmids are often used to clone large DNA fragments (up to about 40 kilobases).
A plasmid into which has been inserted the cos site of bacteriophage. ( 16)
Before YACs were developed, the largest cloning vectors (cosmids) carried inserts of only 20 to 40 kb. (IOOakRidge) Cosmídeo Primeira etapa: Bibliotecas. Diferentes tipos de vetores podendo ser usados. Plasmídios, cosmídeos e plasmídios BAC. (POUniverRJ)
A cloning vector derived from a bacterial virus. It can accommodate about 40 kb of inserted DNA.
An artificial cloning vector (40-50kb of DNA) that can be replicated inside E. coli bacteria.
An artificially constructed cloning vector containing the cos gene of phage lambda.
A plasmid which has had the maximum DNA removed to allow the largest possible insert for cloning but still has the DNA sequences necessary for in vitro packaging into an infective phage particle.
A cloning vector that can replicate autonomously like a plasmid and be packaged into a phage.
A cosmid, first described by Collins and Hohn in 1978, is a type of plasmid (often used as a cloning vector) constructed by the insertion of cos sequences, DNA-Sequences of the Lambda phage.