a bacterial enzyme that breaks DNA at a short, specific sequence. It is also known as a restriction endonuclease.
An endonuclease that recognizes specific nucleotide sequences and cleaves DNA at these sites.
Enzymes that can cut DNA into strands at specific places along its length.
An enzyme normally found in bacteria which cuts DNA at specific sites (i.e. each time a specific nucleotide pattern occurs). Because a restriction enzyme always acts upon DNA in the same manner, a map can be made of a restriction enzymes actions on a known set of nucleotides.
One of a large number of nucleases that can cleave a DNA molecule at any site where a specific short sequence of nucleotides occurs. Extensively used in recombinant DNA technology.
A protein that recognizes specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain over 400 such enzymes that recognize and cut over 100 DNA sequences. Snake venoms are sometimes also used.
An endonuclease which recognizes a specific sequence of bases in a DNA molecule. Type 1 restriction enzymes bind to the recognition site and then cut randomly somewhere along the length of the molecule. Type II restriction enzymes bind at a recognition site and then cleave the molecule by clipping the DNA backbones somewhere within this sequence of bases. Each restriction enzyme has a single, specific recognition sequence, and clips the DNA molecule at a specific site. Thus, treatment of a particular DNA molecule with a particular restriction enzyme will always produce the same set of DNA fragments. It is type II restriction enzymes that have been used extensively in recombinant DNA technology.
Synonym of restriction endonuclease.
An enzyme that cleaves double-stranded DNA at a particular sequence.
A protein that recognizes specific, short nucleotide sequences and cuts DNA at those sites. Over 400 such enzymes that recognize and cut over 100 different DNA sequences have been identified in different species of bacteria.
any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
a molecule that will bind to a particular sequence of DNA bases, then cut the DNA at that point
an enzyme that recognizes a specific substring in the DNA of both prokaryotes and eukaryotes and cuts or cleaves the DNA every place where that pattern occurs exactly where it cuts inside the pattern varies with the pattern
an enzyme that sits down on a DNA strand only when it recognizes a specific sequence of nucleotides
a type of endonuclease
an enzyme that cuts DNA at a restriction site characterized by a specific sequence of nucleotides
More correctly called a Type II Restriction Endonuclease. A bacterial enzyme which forms part of a system to protect the cell against infection by bacteriophage, and unregulated influx of foreign DNA. Binds to DNA at a short specific base sequence, and cuts both strands between specific bases in this sequence.
An enzyme that cuts DNA molecules at a specific base sequence.
A class of enzymes that cut DNA at specific sequences called restriction sites. Restriction enzymes were key to making genetic engineering possible. See RFLP.
An enzyme used to cut DNA at specific sites. The resulting fragments can then be spliced together to form recombinant DNA, which can be separated out on a gel or inserted into a plasmid.
A bacterial endonuclease that recognizes specific base sequences in DNA and breaks the DNA chain at those points.
Restriction enzyme is a shorthand way of saying restriction endonuclease. (Nuclease = an enzyme that cuts a nucleic acid; endo = cuts in the middle, not at the ends; restriction = cutting is restricted to specific sites.) Therefore it is an enzyme that cuts the DNA molecule at specific locations along its length. Each type of restriction enzyme recognises a particular base sequence of the DNA and cuts precisely at the same point each time. (For example, the restriction enzyme EcoR1 recognises the sequence GAATTC, and cuts between the G and its adjacent A. The complementary strand of DNA has the sequence CTTAAG, and here also the enzyme cuts between the A and the G.)
A protein that recognizes a certain sequence of DNA and cuts the DNA at that site.
An enzyme that breaks DNA in highly specific locations, creating gaps into which new genes can be inserted.
an enzyme capable of cutting DNA into fragments.
an enzyme that cuts DNA at specific base pair sequences; this sequence varies from restriction enzyme to restriction enzyme
A bacterial protein that cuts DNA at specific sequences. Hundreds of different restriction enzymes have been discovered so far.
a protein that recognizes specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain over 400 such enzymes that recognize and cut over 100 different DNA sequences. See restriction enzyme restriction enzyme cutting site. [Source: DOE Primer on Molecular Genetics
Enzymes that are capable of recognizing and cleaving specific DNA sequences (usually 4, 6 or 8 bases in length) that result in a single-stranded end. Over 2000 restriction enzymes, with over 200 restriction sites, are known.
Isolated from various bacteria, restriction enzymes recognize short DNA sequences and cut the DNA molecules at those specific sites. (IOOakRidge) Enzima de restrição... foi clivado com a enzima de restrição EcoRI, fracionado por eletroforese em gel de agarose e analisado em ... (POUniverRS)
An endonuclease isolated from bacteria that recognizes and cuts a DNA sequence at a specific sequence. They are used in genetic engineering.
An endonuclease that recognizes specific target nucleotide sequences in DNA and breaks the DNA chain at those points; a variety of these enzymes are known, and they are extensively used in genetic engineering.
An enzyme that cuts DNA at a specific site, allowing biologists to insert or delete genetic material.
enzyme capable of cutting DNA at a specific nucleotide sequence.
A bacterial enzyme which recognizes a specific set of bases and the DNA at that specific site.
A protein which recognizes specific sites on nucleotides or proteins and hydrolyzes the nucleotide or protein at these points.
An enzyme that cuts double-stranded DNA at specific sequences.
Enzyme that recognizes specific nucleotide sequences in DNA and cuts the DNA molecule at these points.
An enzyme that splits a DNA molecule at a sequence of base pairs which is specific to that enzyme. The use of different restriction enzymes will result in different sets of fragments from the same DNA molecule. An extremely important tool in genetic engineering.
endonuclease) Any enzyme that recognizes and cleaves a specific short sequence, the restriction site, in double-stranded DNA molecules. These enzymes are widespread in bacteria and are used extensively in recombinant DNA technology. ( Table 7-1 and Figure 7-5)
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA. The enzyme makes two incisions, one through each of the phosphate backbones of the double helix without damaging the bases. The chemical bonds that the enzymes cleave can be reformed by other enzymes known as ligases, so that restriction fragments carved from different chromosomes or genes can be spliced together, provided their ends are complementary (more below).