variation in the lengths of restriction fragments of DNA. These fragments are obtained from different individuals due to genetic polymorphism of the restriction sites in the chromosomes. It is also known as DNA fingerprinting.
(RFLP) A heritable difference in DNA fragment length and fragment number; passed from generation to generation in a codominant way.
Variation in DNA sequence between individuals that is detectable by variation in the length of DNA fragments generated by digestion with restriction endonucleases.
Restriction fragment length polymorphism (RFLP) is a variation in electrophoresis banding patterns of specific DNA fragments from different individuals of a species. RFLP is caused by the presence of a restriction enzyme cleavage site at one place in the genome of one individual and the absence of that specific site in another individual (a restriction enzyme recognizes a specific target nucleotide sequence in DNA and breaks the DNA chain at that target).
A type of polymorphism or variability in DNA widely used for DNA testing. Based on nucleotide differences that create or abolish cleavage sites for restriction endonucleases.
A variation in DNA sequence that is easily recognized because it occurs at a site where a restriction enzyme cuts a specific sequence, producing DNA fragments of varying lengths. RFLP's often serve as genetic markers.
(Abbreviation: RFLP) A class of genetic marker based on the detection of variation in the length of restriction fragments generated when DNA is treated with restriction endonucleases. Differences in fragment lengths arise due to genetic variation with respect to the presence or absence of specific recognition site(s). RFLPs were initially detected by Southern hybridization but are now detected by electrophoresis of digested PCR product.
(synonym: RFLP) Natural (polymorphic) variation in DNA sequence between individuals which abolishes or creates endonuclease restriction (cutting) sites, resulting in DNA fragments of different lengths when DNA is digested by an endonuclease Related Terms:DNA-based testing ; Southern blot ; molecular genetic testing ; mutation analysis ; polymerase chain reaction (PCR)
Variations between individuals in DNA fragments that are recognised as cutting sites by specific restriction enzymes. Used as markers in chromosome mapping.
A DNA variation that affects the distance between restriction sites (most often by a nucleotide change that creates or eliminates a site) within or flanking a DNA fragment recognized by a cloned probe (see Chapter 8). RFLPs are detected upon Southern blot hybridization. The term RFLP is commonly used even in situations where the DNA variation may not represent a true polymorphism in the population-based definition of this term. See RFLP in the MGI Glossary.
a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. For example, DNA from P. stutzeri KC has a unique RFLP pattern that differentiates it from other isolates in the aquifer.
A change in the DNA of an organism that changes how a restriction enzyme cuts the DNA into pieces. Two or more organisms can be compared based on the pattern of their DNA fragments when they are run on a gel (by electrophoresis). If the organisms have different spaces between their restriction enzyme sites, they produce different lengths of fragments when the restriction enzyme is used to cut up the DNA. When their fragments are run on a gel, they produce different patterns because the shorter fragments will move further than the longer ones.
The detection of variations in DNA sequence in populations by cleaving DNA with restriction enzymes and analyzing the patterns of the resulting DNA fragments.
Variation in the structure of DNA from person to person that is demonstrated by the cutting of DNA into fragments of differing length by means of restriction enzymes.
Variation in the distance between restriction enzyme cleavage sites that exist within a population producing unique DNA fingerprint patterns.
See single nucleotide polymorphism.
(RFLP) Genetic variations at the site where a restriction enzyme cuts a piece of DNA. Such variations affect the size of the resulting fragments. These sequences can be used as markers on physical maps and linkage maps. RFLP is also pronounced "rif" lip.
Variation in the length of a stretch of DNA.
(RFLP) The occurrence of variation in the length of DNA fragments that are produced after cleavage with a type II restriction endonuclease. The differences in DNA lengths are due to the presence or absence of recognition site(s) for that particular restriction enzyme. RFLPs were initially detected using hybridization with DNA probes after separation of digested genomic DNA by gel electrophoresis (Southern analysis). Now they are typically detected by electrophoresis of digested PCR product.
A mutation that results in a detectable change in the pattern of fragments obtained when a DNA molecule is cut with a restriction endonuclease.
RFLP) The first method of DNA profiling used in casework in Victoria. It involves cutting up the DNA into small fragments, running them on a gel and applying a probe which binds to the DNA
Variation between individuals in DNA fragment sizes cut by specific restriction enzymes; polymorphic sequences that result in RFLPs are used as markers on both physical maps and genetic linkage maps. RFLPs are usually caused by mutation at a cutting site. See marker.
Examples of these types of markers include (1) restriction fragment length polymorphisms (RFLPs), which reflect sequence variations in DNA sites ... (IOOakRidge) Polimorfismo de tamanho de fragmentos de restrição... quanto a possíveis polimorfismos de tamanho de fragmentos de restrição (RFLP) EcoRI no genoma deste carrapato. (POUniverRS)
Differences in DNA sequence on homologous chromosomes that result in restriction fragments of varying lengths that can be detected using DNA probes.
refers to inherited differences in sites for restriction enzymes between two related DNA sequences (for example, caused by base changes in the target site) that result in differences in the lengths of the fragments produced by cleavage with the relevant restriction enzyme. RFLPs are used for genetic mapping to link the genome directly to a conventional genetic marker.
A technique used in the process of DNA profiling.
Variations in the size of DNA fragments produced by a restriction endonuclease at a polymorphic locus.
In molecular biology, the term restriction fragment length polymorphism (or RFLP, often pronounced "rif-lip") is used in two related contexts: as a characteristic of DNA molecules (arising from their differing nucleotide sequences) by which they may be distinguished, as the laboratory technique which uses this characteristic to compare DNA molecules. The technique is utilized in genetic fingerprinting and paternity testing.