The use of phenotype or protein information to identify the gene encoding the protein.
The experimental procedure that begins with a cloned segment of DNA, or a protein sequence, and uses this knowledge to introduce programmed mutations (through directed mutagenesis) back into the genome in order to investigate gene and protein function.
An experimental approach that begins with information about the primary DNA or protein sequence and uses this knowledge to generate targeted mutations (heritable changes) or altered expression levels. Observation of the resulting effects on the organism (a physical attribute such as the color of the flower or the shape of the leaf) yields information about the physiological function of the gene or protein. This is the reverse strategy to a classical genetics approach that proceeds from observing a defined genetic trait towards obtaining sequence information for a specific gene.
An approach where a cloned gene with an unknown function is used to disrupt the corresponding chromosomal gene to examine the resulting phenotype.
A genetic approach that begins with discovery of a gene (or its DNA sequence) and then its uncovers its biological function.
Use positional cloning.
An old name for positional cloning.
The experimental procedure that begins with a cloned segment of DNA or a protein sequence and uses it (through directed mutagenesis) to introduce programmed mutations back into the genome to investigate function.
Reverse genetics is an approach to discovering the function of a gene that proceeds in the opposite direction of so called forward genetic screens of classical genetics. Simply put, while forward genetics seeks to find the genetic basis of a phenotype or trait, reverse genetics seeks to find the possible phenotypes that may derive from a specific genetic sequence enumerated during DNA sequencing.