empty volume in a chromatogram representing tubing between the injector and column or between column and detector; an excess can cause band broadening by diffusion.
Any volume which a component introduces to a system that is not cleanly swept and relies on diffusion to clear the space. See connecting volume.
The volume inside the pressure port of a sensor, or transducer, at room temperature and barometric pressure.
The volume outside of the column packing itself. The interstitial volume (intraparticle and interparticle volume) plus extracolumn volume (contributed by injector, detector, connecting tubing, and endfittings) all combine to create the dead volume. This volume can be determined by injecting an inert compound. For example, a compound that does not interact with the column packing. It is also abbreviated Vo or Vm.
The volume of the pressure port of a transducer at room temperature and ambient barometric pressure.
That portion of the flow path that is not directly Inline, that is unswept. These small spaces within the LC system allow remixing of the separated sample bands, as well as dilution of the initial sample with mobile phase. Dead volume should be minimized in a LC system, especially when small volume columns of high plate number are used.
