Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
A method that uses the reaction of antibodies with cell antigens to determine a specific type of cell in a sample of blood cells, marrow cells, or lymph node cells. The antibodies react with specific antigens on the cell. A tag is attached to antibody so that it can be detected. The tag can be identified by the laboratory equipment used for the test. As cells carrying their array of antigens are tagged with specific antibodies they can be identified; for example, myelogenous leukemic cells can be distinguished from lymphocytic leukemic cells. Normal lymphocytes may be distinguished from leukemic lymphocytes. This method also helps to subclassify cell types, which may, in turn, help to decide on the best treatment to apply in that type of leukemia or lymphoma. The antigen on a cell is referred to as cluster of differentiation or “CD” with an associated number. For example, CD16 may be present on leukemic lymphoblasts and CD33 on leukemic myeloblasts.
Determining what kind of surface molecules are present on cells. Used by pathologists to determine the exact type of leukemia from a blood sample.
identifying cells based on the types of antigens or markers on the surface of the cell to diagnose the subtype of AML
A process used to identify cells, based on the types of antigens or markers on the surface of the cell. This process is used to diagnose specific types of leukemia and lymphoma by comparing the cancer cells to normal cells of the immune system.
A test that can identify specific cells by monitoring the reaction between antibodies and antigens. This can help them determine the origin of the malignant cells.
Immunophenotyping is a technique notably used in the diagnosis of leukemia. It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane. By choosing appropriate antibodies, the origin of leukemic cells can be accurately determined.
