A technique that identifies genes based on their position on the chromosome. Positional cloning is accomplished by using linkage analysis to locate a mutation relative to a known genetic marker, followed by chromosomal jumping and walking to screen for po
A process which, through gene mapping techniques, is able to locate a gene responsible for a disease when little or no information is known about the biochemical basis of the disease.
Identification of a gene based on its physical location in the genome. Often, an individual has a phenotype, but the gene underlying this phenotype is unknown. Using linkage mapping, the phenotype can be assigned a position in the genome. Once a phenotype has been localized, overlapping sets of clones (for example, BACs) that cover the region are identified. Genes within the region are identified and compared to DNA from individuals that display the phenotype until the underlying mutation is identified. Read more about positional cloning references: Kerem B, et al. Identification of the cystic fibrosis gene: genetic analysis. Science 1989; 8;245(4922):1073-80.
Locating the gene for a specific trait by comparing the DNA of people who have that trait to the DNA of relatives who don't have the trait.
A technique to identify genes, usually those associated with diseases, based on their location on a chromosome. A procedure that uses information on the map position of a gene to obtain a clone of that gene.
Requires a genetic map with a large number of markers (especially in the region of interest), and the use of physical mapping and DNA sequencing technologies to isolate and sequence the targeted gene.
A strategy for the identification of disease genes on the basis of marker inheritance data from affected families that does not require any prior knowledge of the underlying biological pathways or gene function (in contrast to 'candidate-gene' approaches). In recent years, a blend of positional cloning and candidate-gene approaches (sometimes referred to as a 'positional-candidate' strategy) has often been used, involving the combined use of data on map location and expected gene function.
Isolation and cloning of the normal form of a mutation-defined gene (i.e., a gene identified by genetic analysis of mutants).
Identification of a gene based on its location in the genome. --Click Here For Details
The localization of a gene to a particular region of a chromosome which then leads to its isolation.
(synonym: reverse genetics) The cloning or identification of a gene for a particular disease based on its location in the genome, determined by a collection of methods including linkage analysis, genomic (physical) mapping, and bioinformatics, when no information about the biochemical basis of the disease is known; distinguished from the more common strategy of gene cloning beginning with a known protein product, determining its amino acid sequence, and using that information to isolate the gene
The cloning of a gene on the basis of its chromosomal location rather than its functional properties. Also called reverse genetics.
Method used to define the location of a gene on a chromosome and use this information to identify and clone the gene. The location of the gene is determined by linkage analysis of DNA from a large family containing afflicted and normal members to identify linkages between the transmission of the disease gene and observable genetic markers. This information is then used to screen (by chromosomal jumping and walking) the location for putative genes. The disease gene must be compared between the afflicted and normal family members and be shown to be different in the two groups. The full sequencing of the gene will then provide information regarding the characteristics and function of the gene product, and a potential explanation for the cause of the disease.
A technique used to identify genes, usually those that are associated with diseases, based on their location on a chromosome. Source : Human Genome Project Information
the process of finding the gene for a specific trait by comparing the DNA of people with the trait to the DNA of relatives without the trait.
A technique used to identify genes, usually those that are associated with diseases, based on their location on a chromosome. This is in contrast to the older, "functional cloning" technique that relies on some knowledge of a gene's protein product. For most diseases, researchers have no such knowledge.
Aims to identify the locus and the gene responsible for a particular disorder/protein relative to mapped markers, usually microsatellites. This is determined by linkage analysis which assesses the frequency with which a particular marker cosegregates with the disorder/protein in families. Because there may be crossing over of DNA between a pair of chromosomes the further a gene is from a marker the lower the chance of cosegregation. Cloning involves sequencing the gene in its entirety, usually in overlapping segments.