Definitions for "Southern blot"
An experimental technique designed to identify and quantitate DNA.
(SB) describes the technique first developed by the Scottish molecular biologist Edward M. Southern which now bears his name. Specimen DNA is denatured, treated with restriction enzymes to result in DNA fragments and then the single-stranded DNA fragments are separated by electrophoresis. The electrophoretically separated fragments are then blotted to a nitrocellulose membrane, retaining their electrophoretic position, and hybridized with radiolabeled single- stranded DNA fragments with sequences complementary to those being sought. The resulting double-stranded DNA bearing the radiolabel is then, if present, detected by autoradiography.
a DNA technology that uses a radioactive probe to match with specific DNA fragments