Definitions for "Expression vector"
Related Terms:
Exons, Operator, Klenow fragment, Promoter, Genomic dna, Cloning vector, Replicon, Origin of replication, Rdna, Homeobox, Cosmid, Transposase, Restriction endonuclease, Okazaki fragment, Pseudogene, Restriction enzyme, Plasmid, Introns, Bac, Intron, Restriction fragment, Recombinant dna, Pseudogenes, Bacterial artificial chromosome, Amplicon, Replisome, Restriction fragment length polymorphism, Dna sequence, Homologous recombination, Plasmids, Yac , Gene cloning, Insertion, Molecular marker, Recombinant dna technology, Southern blot, Repressor, In situ hybridization, Dna probes, Polymerase chain reaction, Exon, Restriction site, Repetitive dna, Dna probe, Primosome, Yeast artificial chromosome, Rflp, Transcription factors, Expressed sequence tag, Est
A cloning vector that contains the necessary regulatory sequences to allow transcription and translation of a cloned gene or genes.
A modified plasmid or virus that carries a gene or cDNA into a suitable host cell and there directs synthesis of the encoded protein. Some expression vectors are designed for screening DNA libraries for a gene of interest ( Figures 7-21 and 20-9); others, for producing large amounts of a protein from its cloned gene ( Figures 7-36 and 7-37).
virus or plasmid that harbours a DNA sequence constructed in the aim of expressing a specific protein into a host cell.
A virus or plasmid that carries a DNA sequence into a suitable host cell and there directs the synthesis of a specific protein.
A vector that has been designed to express cloned genes in a particular cell type.
In recombinant DNA work, a type of vector that contains promoters and other regulatory sequences that enable a protein-coding DNA inserted into the vector to be transcribed and translated if introduced into the appropriate cells.
DNA vector, such as a plasmid, that carries a DNA sequence that includes the adjacent sequences for its expression into mRNA and protein in a host cell.
Plasmid or virus vector that carries into a cell a DNA sequence that directs the synthesis of a protein.
a DNA molecule comprising a gene that is expressed in a host cell
a DNA molecule, linear or circular, that comprises a DNA segment encoding a foreign DNA of interest operably linked to additional segments that provide for its transcription
a nucleic acid construct, generated recombinantly or synthetically, bearing a series of specified nucleic acid elements that enable transcription of a particular gene in a host cell
a nucleic acid construct, typically generated recombinantly or synthetically, with a series of specified nucleic acid elements which permit transcription of a particular nucleic acid in a host cell
a plasmid derived construction designed to enable the expression of a gene carried on the vector
a relatively small DNA molecule that can be used to carry a specific gene into a target cell
a replicable DNA construct in which a DNA sequence encoding a protein or peptide as given herein is operably linked to suitable control sequences capable of effecting the expression of the DNA sequence in a suitable host
a vector which (due to the presence of appropriate transcriptional and/or translational control sequences) is capable of expressing a DNA (or cDNA) molecule which has been cloned into the vector and of thereby producing a polypeptide or protein
A vector which is designed to allow expression (transcription and translation) of the inserted section of DNA. The vector carries a promoter (normally inducible) on one side of the cloning site, and a transcription terminator on the other side. These must be recognisable by the intended host cell. The vector may also carry a ribosome binding sequence (for bacterial expression) and a start codon, depending on the nature of the inserted DNA. Some expression vectors produce fusion proteins.
A way of delivering foreign genes to a host, creating a recombinant organism that will express the desired protein.
A type of cloning vector that enables the foreign gene it carries to be expressed as polypeptide in the recipient host cell.
A cloning vector that is engineered to allow the expression of protein from a cDNA. The expression vector provides appropriate promoter and restriction sites that allow insertion of cDNA.
Typically a small, circular piece of DNA that is transformed (inserted) into a particular expression host for the purpose of producing the protein coded for by the DNA.
The vector into which the gene of interest has been cloned.
A cloning vector that contains regulatory sequences that promote transcription and translation of a cloned gene.
is a cloning vector designed so that a coding sequence inserted at a particular site will be transcribed and translated into protein.
A cloning vector designed so that a foreign gene inserted into the vector will be expressed in the host organism.
An expression vector is a relatively small DNA molecule that is used to introduce and express a specific gene into a target cell. Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular transcription and translation machinery.