'Expressed Sequence Tags' provided by the Japanese cDNA Project and published in GenBank. All ESTs in dictyBase have a locus page.
cDNA sequence of a few hundred bases used to identify the corresponding gene.
Expressed Sequence Tag. A partial sequence of a clone, randomly selected from a cDNA library and used to identify genes expressed in a particular tissue. ESTs are used extensively in projects to map the human genome.
Expressed sequence tag. These are single-pass sequences of cDNA clones. Databases of EST sequences are highly redundant but quite useful for gene identification. There are many efforts to cluster EST sequences to remove the redundancy and low-quality sequences. EST clusters in UniGene Gene indexes at TIGR EST clusters at Allgenes references: Adams MD et al. Complementary DNA sequencing: expressed sequence tags and human genome project. Science 1991; 252(5013):1651-6. Marra M et al. An encyclopedia of mouse genes. Nat Genet 1999; 21(2):191-4.
Expressed Sequence Tags. Cloned gene fragments that result from an active gene during a certain stage of a cell or organ
expressed sequence tag. a sequence from a transcribed mRNA for a possibly unknown gene. Individual EST's are neither especially informative nor highly accurate; however, they are easy to collect in large numbers. A database of EST's can be helpful for picking protein-coding sequences out of a long stretch of DNA, or for providing a larger context for very short sequences, such as the small pieces of protein sequence obtainable by mass spectrometry. EST's are compiled by the National Center for Biotechnology Information in a database called dbEST.
Expressed sequence tag. ESTs are usually short (300-500 hp) single reads from mRNA (cDNA) which are usually produced in large numbers. They represent a snapshot of what is expressed in a given tissue, and/or at a given developmental stage. They represent tags (some coding, others not) of expression for a given CDNA library. (See: Expressed Sequence Tag).
Expressed Sequence Tag; a sequenced cDNA that can be used as a marker for genetic mapping.
Expressed sequence tag. A unique segment of cDNA with a base sequence identical to at least part of the coding region of a gene, generally used as landmark for mapping.
Expressed sequence tag. See sequence tagged site.
(Expressed Sequence Tag) Small sequence from an expressed gene that can be amplified by PCR. Act as physical markers for cloning and full length sequencing of the cDNAs of expressed genes. Typically identified by purifying mRNAs, converting to cDNAs, and then sequencing a portion of the cDNAs
Expressed sequence tag. tags of expressed sequence that can be used as landmarks along the human genome and to provide clues about the location of new and similar genes.
expressed sequence tag. a short strand of DNA (approximately 200 base pairs long) which is part of a cDNA. Because an EST is usually unique to a particular cDNA, and because cDNAs correspond to a particular gene in the genome, ESTs can be used to help identify unknown genes and to map their position in the genome.
Expressed Sequence Tag. A short DNA sequence from a coding region that is used to identify a gene.
Expressed sequence tag. A known cDNA sequence of several hundred nucleotides, which forms part of a gene and is derived from RNA. The RNA usually codes for a protein or protein fragment of unknown function.
A partial sequence of a cDNA clone that can be used to identify sites in a gene.
Expressed Sequence Tag; a sampling of sequence from a cDNA.
Expressed sequence tag. Partial gene sequence data of a cDNA clone which provides a sequence tag for a gene. In order to achieve a very high throughput, these sequences are usually only subjected to a single-pass of sequencing so the error rate in these sequences can be high, perhaps approaching 5%.
Expressed sequence tag. A partial sequence of an expressed gene. EST sequence is obtained by high- throughput methods and is useful in the mapping of genomes.