enzymes produced in various bacterial species that cleave DNA molecules at specific nucleotide sequences. Used in recombinant DNA technology. Named for their role in restricting the host range of bacteriophage.
Enzyme that breaks nucleic acid (usually DNA) at only one specific sequence of nucleotides.
A class of enzymes that cut DNA after recognizing a specific sequence. The three types of restriction endonuclease are: I. Where the cut occurs within a random sequence at sites 1kbp from the recognition sequence, and has both restriction and methylation activities. II: Cuts within, or near a short, usually palindromic recognition sequence. A separate enzyme methylates the same recognition sequence. III: Cuts 24-26bp downstream from a short, asymmetrical recognition sequence, requires ATP and has both restriction and methylation activities. Type II enzymes are the class used for most molecular biology applications.
( restriction enzyme) An enzyme that hydrolyzes internal phosphodiester bonds in the DNA.
any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
an enzyme that breaks bonds only within a specific sequence of bases
An enzyme that cleaves DNA at a specific site.
An enzyme with the ability to recognize a particular nucleotide sequence and to cleave a DNA molecule at that point.
An enzyme, isolated from bacteria, that recognizes specific base-pair sequences within DNA and causes endonucleolytic cleavage of the DNA at a site determined by the recognized DNA sequences. The sites vary from frequent to rare cutting, depending upon the length of the restriction site.
an enzyme able to cleave the phosphodiester bonds within a nucleic acid strand by binding to DNA at a specific site.
a class of enzymes which recognize a specific base sequence (usually four to six base pairs in length) in a double-stranded DNA molecule and cuts both strands of the DNA at every site where this sequence occurs.
An enzyme-produced bacteria which cleaves or cuts DNA into fragments at specific sites.
Any one of several enzymes, produced by bacteria, that break foreign DNA molecules at very specific sites. Some produce "sticky ends." Extensively used in recombinant DNA technology.
An endonuclease cuts double stranded DNA by binding to specific sites, in many cases arranged in palindromes. Several different classes of restriction endonucleases can be differentiated based upon their binding and cleavage sites and their required cofactors. Although restriction endonucleases have specific recognition sites, cleavage may occur at specific or random sites depending on the class of the endonuclease. Also called restriction enzymes.
an enzyme which cuts the DNA molecule in highly specific locations. For example, the restriction enzyme known as Bam H1 cuts DNA only at a specific 6-base sequence.
An enzyme that cuts DNA at specific nucleotide sequence; functions as a protective mechanism and a research tool.
An endonuclease that binds to double-stranded DNA at a specific nucleotide sequence and then, if both strands of the DNA lack appropriate modification at that sequence, cleaves the DNA either at the recognition sequence or at another site in the DNA molecule. ( 16)
