The transfer of a cell nucleus from a somatic cell into an egg from which the nucleus has been removed.
The transfer of a cell nucleus from a somatic cell into an egg (oocyte) whose nucleus has been removed.
(SCNT) Transfer of the nucleus of a somatic cell into a secondary oocyte or zygote from which the egg or zygote's chromosomes have been removed, in such a way that cleavage still occurs, in effect causing the cell's nucleus to 'bud', or reproduce without genetic recombination, and hence a form of asexual reproduction or cloning now commonly used for reproductive cloning in animals and, it is hoped, for therapeutic cloning to create embryonic stem cells for treatment of serious degenerative disease and cancers.
Transfer of the nucleus from a donor somatic cell to an unfertilized egg cell from which the maternal chromosomes have been removed.
The technical name for the method used to produce the first animal clone, a sheep called "Dolly." Though the name suggests that a nucleus from a somatic cell was used, in fact, the complete somatic cell was fused with an enucleated egg.
moving a cell nucleus and its genetic material from one cell to another
Technique in which a nucleus is implanted into an enucleated ovum and the resulting egg is allowed to develop to produce a cloned organism
A cloning technique where the nucleus from an unfertilized egg is removed and replaced with the nucleus from a somatic cell. The resulting egg will carry the full complement of genetic material of the host organism. This is how Dolly the cloned sheep was produced; she was genetically identical to her "mother". This technique can be used both for reproductive cloning and therapeutic cloning.
also known as cloning, a procedure where the nucleus of an egg is removed and the nucleus of a somatic cell is placed in the egg. The egg is then "shocked" and begins to grow as if it were fertilized. The resulting embryo is a genetic clone of the donor of the somatic cell. Often called therapeutic cloning.
Process in which the nucleus containing half a genome is removed from a human egg cell and replaced with a nucleus containing a full genome from an adult cell of any kind from a donor. The cytoplasm within the egg awakens, or turns on, the full DNA strand in the nucleus, allowing it to begin to direct cell division. The result is a set of totipotent stem cells that soon begin to specialize into pluripotent, and then, within two weeks, multipotent stem cells.
the replacement of genetic material (nuclear DNA) in an unfertilized egg with genetic material from an adult somatic cell (e.g., skin cell). Stem cells that genetically match the adult somatic cell donor can be derived. See graphic.
The transfer of a cell nucleus from a body cell into an egg from which the chromosomes have been removed or inactivated; method used for cloning of an organism. Once the transferred genome is within the egg cell and a one-cell embryo is created, the process of cloning is complete and further development of the clone can occur.
See definition for nuclear transfer.
Placing a cell nucleus into an egg whose own nucleus has been stripped out, and “tricking” the egg into thinking it has been fertilized in order to make the cell divide.
a process whereby the nucleus of a somatic cell is removed and placed into an enucleated oocyte (i.e. an egg cell that has had its own nucleus i.e. all genetic information removed). The net result is to have the genetic information from the donor nucleus in control of the resulting cell. With further manipulation, such cells can be induced to form embryos. This process was used to create the cloned sheep "Dolly".
A method of cloning: transfer of the nucleus from a donor somatic cell into an enucleated egg to produce a cloned embryo.
Sometimes known as therapeutic cloning. A process by which a nucleus from a single cell (for example a skin cell) is transferred into an unfertilized egg, from which the nucleus (the genetic contents) have been removed. The resulting reconstructed embryo is which is then allowed to develop to the blastocyst stage. Embryonic stem cells derived from this blastocyst are genetically identical to the donor of the original nucleus.
Technique by which a somatic cell nucleus is transplanted into an ovum whose own nucleus has been removed (enucleated). Process also referred to as nuclear transfer or transplantation.
In SCNT, a nucleus from a patient's body cell, such as a skin cell, is introduced into an unfertilized egg from which the original genetic material has been removed. The egg is then used to produce a blastocyst whose stem cells could be used to create tissue that would be compatible with that of the patient. This is called therapeutic cloning. Its advocates say the process does not result in the creation--or destruction--of human life because the egg is never fertilized or implanted in a uterus, as it would be in reproductive cloning.
A technique in which the nucleus of any cell of the body (somatic cell) - other than sperm or egg - is injected into an egg that has had its nucleus removed. The nucleated egg is then electrically stimulated, prompting it to take on the genetic and molecular characteristics of an embryo. The embryonic stem cells taken from the embryo in the culture dish will be genetically identical to the body cell from which the nucleus was derived. The technique could prove useful for cell transplantation therapy, as the embryonic stem cells offer a way of providing genetically identical cells to patients who donate the nuclei, thus avoiding the hurdle of immune system rejection.
In genetics and developmental biology, somatic cell nuclear transfer (SCNT) is a laboratory technique for creating an ovum with a donor nucleus (see process below) . It can be used in embryonic stem cell research, or in regenerative medicine where it is sometimes referred to "therapeutic cloning." It can also be used as the first step in the process of reproductive cloning.