A technique used to detect differences in proteins and polypeptide chains based on differences in the size and electrical charges of the molecules.
(jel ul lec tro for´ eesis) • A semisolid matrix suspended in a salty buffer in which molecules can be separated on the basis of their size and change when current is passed through the gel.
method to separate out DNA or proteins or RNA of different sizes or charges, by applying an electric current
A technique where nucleic acids or proteins are separated according to size and charge by subjecting them to an electric current in a suitable gel and buffer system.
A method of separating large molecules (such as DNA fragments or proteins) from a mixture of similar molecules. An electric current is passed through a medium containing the mixture, and each kind of molecule travels through the medium at a different rate, depending on its electrical charge and size.
A technique used to separate molecules according to size or charge. The molecules are passed through a gel under the influence of an electric field.
The separation and identification of molecules based on their movement through an electrically charged field.
electrophoresis in which the supporting medium is a gel (usually agarose or acrylamide); used for separating proteins and nucleic acids base on net charge and size.
Electrophoresis performed in a gel matrix so that molecules of similar electric charge can be separated on the basis of size.
so that the specific base at the end of each successive fragment is detectable after the fragments have been separated by gel electrophoresis. (IOOakRidge) Eletroforese em gel... fracionado por eletroforese em gel de agarose e analisado em "Southern blottings" com as sondas acima mencionadas. (POUniverRS)
A technique used to separate molecules according to their sizes and charges.
a DNA separation technique that is very important in DNA sequencing. Standard sequencing procedures involve cloning DNA fragments into special sequencing cloning vectors that carry tiny pieces of DNA. The next step is to determine the base sequence of the tiny fragments by a special procedure that generates a series of even tinier DNA fragments that differ in size by only one base. These nested fragments are separated by gel electrophoresis, in which the DNA pieces are added to a gelatinous solution, allowing the fragments to work their way down through the gel. Smaller pieces move faster and will reach the bottom first. Movement through the gel is hastened by applying an electrical field to the gel.
Gel electrophoresis is the process of separating a mixture of molecules in a gel media by the application of an electric field. In general, molecules with similar electric charges and density will migrate at the same rate together in the gel.
A research technique used to separate molecules (or fragments of a molecule) according to size. Upon electrical stimulation, smaller fragments of a molecule will move faster through the gel than larger fragments. The process is typically done to separate DNA fragments after the DNA has been cut with restriction enzymes.
A method for separating nucleic acids or proteins based on size through a gel matrix. The movement through the matrix is induced by an electrical current that is applied to the gel, the separation results from the tunable resistance provided by the gel.
A type of electrophoresis in which the molecules in a sample moves through a gel composed of agarose or polyacrylamide. ( 16)
Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point. Gel electrophoresis is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules prior to use of other methods such as mass spectrometry, PCR, cloning, DNA sequencing, or immuno-blotting for further characterization.