A procedure in which a mixture of proteins is separated on a polyacrylamide gel and then transferred to a nylon membrane. The membrane may then be treated with reagents such as specific antibodies to locate a protein of interest.

Similar to Southern blot, except that antigenic proteins of an organism are separated by gel electrophoresis and transferred to membrane filters. Antiserum is allowed to react with the filters, and specific antibody bound to its homologous antigen is detected using labeled anti-antibody detectors. See immunoblot.

A technique for analysing mixtures of proteins to show the presence, size and abundance of one particular type of protein. Similar to Southern or Northern blotting (see "Blotting"), except that (1) a protein mixture is electrophoresed in an acrylamide gel, and (2) the "probe" is an antibody which recognises the protein of interest, followed by a radioactive secondary probe (such as 125I-protein A).

A blood test that detects HIV-specific antibodies. If the test is positive, the subject has been infected by the virus. This test is more accurate than the ELISA test.

A technique in which protein is transferred from an electrophoretic gel to a cellulose or nylon support membrane following electrophoresis. A particular protein molecule can then be identified by probing the blot with a radiolabelled antibody which binds only the specific protein to which the antibody was prepared. Useful, for example, for measurement of levels of production of a specific protein in a particular tissue or at particular developmental stage. cf  blot; northern blot; Southern blot.

A useful tool for characterizing proteins in a biological sample. In this technique, proteins are first separated by size using SDS-PAGE. The contents of the gel are transferred to a membrane, and then probed with a series of antibodies to find the protein of interest. See also Antibodies, SDS-PAGE.

A test for specific antibodies to confirm repeatedly reactive results on the ELISA or EIA test.

a test used to see if the patient has HIV Disease. It is a blot consisting of a sheet of cellulose containing spots of protein for indentification by a molecular probe. This test looks for HIV antibodies -- a protein produced by the immune system as it tries to fight the HIV virus.

Technique for detecting specific proteins separated by electrophoresis by use of labelled antibodies.

a method in molecular biology to detect a certain

a method in molecular biology todetect a certain protein in a sample by using antibody specific to that protein

a method in molecular biology to detect specific proteins

a metod in molecular biology to detect a certain protein in a sample by using antibody specific to that protein

a more expensive and definitive test

A confirmation test for the presence of specific antibodies that is more accurate than the ELISA test for detecting true negatives.   Click here to return to list  Click here to return to list  Click here to return to list

follow-up blood test for anti- Borrelia burgdorferi antibodies. This test is used to confirm the results of an ELISA test.

a laboratory blood test for specific antibodies (e.g., to HIV). The Western blot assay is more accurate than the ELISA, and is used as a confirmatory test if an ELISA is positive.

Technique used to confirm presence of a disease; it identifies the presence in the blood of antibodies to proteins of specific size. WI-38 A human diploid cell strain developed in 1961 by Leonard Hayflick.

A technique whereby a complex mixture of size-separated proteins is fixed to a solid support, and then probed with a labelled antibody. Useful, for example, for the measurement of levels of production of a specific protein in a particular tissue or at particular developmental stage.

A blood test that detects antibodies to HIV-1. Used to confirm EIA results.

this is a confirmatory blood test for HIV infection that is done to make absolutely sure that a person has HIV

A technique developed in 1979 that is used to confirm ELISA results. HIV antigen is purified by electrophoresis and attached by blotting to a nylon or nitrocellulose filter. The patient's serum is reacted against the filter, followed by treatment with developing chemicals that allow HIV antibody to show up as a colored patch or blot. If the patient is HIV-positive, there will be stripes at specific locations for two or more viral proteins. A negative result is blank.

One of the major confirmatory tests for HIV antibodies in the blood (see Elisa).

Technique in which proteins are separated via electrophoresis and then immobilized on a membrane for further analysis.

An electrophoresis-based technique used to find proteins based on their ability to bind to specific antibodies.

A test for detecting the specific antibodies to HIV in a person's blood. It commonly is used to double-check positive ELISA tests (see). A western blot test is more reliable that the ELISA, but it is harder to do and costs more money.

A protein blot. (This term originated as lab jargon; not a generally accepted term.) ( 19)

Laboratory test used to confirm results on the HIV ELISA or EIA tests. In the U.S., Western Blot is the validation test used most often for confirmation of these other tests.

A blood test for HIV antibodies.

A western blot (a.k.a immunoblot) is a method in molecular biology/biochemistry/immunogenetics to detect protein in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate denatured proteins by mass. The proteins are then transferred out of the gel and onto a membrane (typically nitrocellulose), where they are "probed" using antibodies specific to the protein.