A vector capable of replication in bacteria that is used to clone 100 to 300 kb DNA fragments into E. coli cells.
Cloning vector that can incorporate large fragments of DNA. (see YACS)
(BAC). A cloning vector derived from a single-copy F-plasmid of Escherichia coli that carries the F replication and partitioning systems that ensure low copy number and faithful segregation of plasmid DNA to daughter cells. Large genomic fragments can be cloned into such vectors and they are faithfully replicated, which makes BACs useful for constructing genomic libraries.
Method of construction of genomic library, in which the vector contains sites necessary for the DNA to be handled and replicated as a bacterial chromosome, allowing clones to contain very large pieces of DNA (around 200 kb).
An artificial chromosome created from modification of the fertility factor of plasmids which allows incorporation of up to 330 kb of foreign DNA.
(BAC) A circular piece of DNA that can exist and replicate within bacteria. Often used to clone DNA fragments in Escherichia coli cells.
(BAC) Large segments of DNA, 100,000 to 200,000 bases, from another species cloned into bacteria. Once the foreign DNA has been cloned into the host bacteria, many copies of it can be made.
a vehicle based on the bacteria E. coli that is used to copy, or clone, fragments of DNA that are 150,000 to 180,000 base pairs (bp) long. These DNA fragments are used as starting material for DNA sequencing.
A chromosome-like structure constructed using recombinant-DNA technology. It is used to clone large DNA inserts (100 to 300 kb) into E. coli cells.
A vector used to clone DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli cells. Based on naturally occurring F-factor plasmid found in the bacterium E. coli.
A long sequencing vector which is created from a bacterial chromosome by splicing a DNA fragment of 100kb (or more) from another species. Once the foreign DNA has been cloned into the host bacteria, many copies of the new chromosome can be made.
A bacterial artificial chromosome (BAC) is a DNA construct, based on a fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. Its usual insert size is 150 kbp, with a range from 100 to 300 kbp. A similar cloning vector, called a PAC has also been produced from the bacterial P1-plasmid.