An experimental technique designed to identify and quantitate DNA.
(SB) describes the technique first developed by the Scottish molecular biologist Edward M. Southern which now bears his name. Specimen DNA is denatured, treated with restriction enzymes to result in DNA fragments and then the single-stranded DNA fragments are separated by electrophoresis. The electrophoretically separated fragments are then blotted to a nitrocellulose membrane, retaining their electrophoretic position, and hybridized with radiolabeled single- stranded DNA fragments with sequences complementary to those being sought. The resulting double-stranded DNA bearing the radiolabel is then, if present, detected by autoradiography.
a DNA technology that uses a radioactive probe to match with specific DNA fragments
DNA blot taken from an electrophoresis gel. The original blot type named after its originator Ed Southern
(synonyms: Southern analysis, Southern blot analysis, Southern blotting, Southern blotting analysis) Molecular genetic testing technique used to detect differences in the lengths of DNA fragments following restriction enzyme digestion (commonly known as RFLPs: restriction fragment length polymorphisms). The lengths of restriction fragments can vary due to mutations occurring between two restriction sites (e.g., large insertions, large deletions, and highly expanded trinucleotide repeats) or within a restriction site (e.g., single base pair changes). Related Terms: direct DNA analysis ; molecular genetic testing ; mutation analysis ; restriction fragment length polymorphism analysis ; restriction site
The transfer of size-separated DNA fragments to a synthetic membrane for further studies, initially described by E.N. Southern.
A technique that utilizes labeled DNA probes to identify DNA fragments that have been transferred to membrane filters following electrophoretic separation.
Procedure which transfers elecrophoretically separated DNA fragments on an agarose gel to nitrocellulose filters for detection by hybridization with a labeled probe complementary to the sequence of interest; the position on the filter of the probe, when exposed to x-ray film, appears as a band on an autoradiogram.
A technique used to identify and locate DNA sequences which are complementary to another piece of DNA called a probe.
DNA from a sample is cut with restriction enzymes and the position of the fragments (e.g. on a gel) is determined by the fragment's molecular weight. Complementary strands of radioactively labelled DNA are used to identify the position of the DNA fragments on the gel.
transfer of DNA molecules separated by size, from a gel to a flexible membrane (nitrocellulose or nylon).
A method in which DNA fragments are separated via electrophoresis and then immobilized on a membrane for further analysis.
An electrophoresis-based technique used to find DNA sequences which are complementary to a DNA probe.
Transfer of electrophoretically separated fragments of DNA from the gel to an absorbent sheet such as paper. This sheet is then immersed in a solution containing a labeled probe that will bind to a fragment of interest.
A Southern blot is a method in molecular biology of enhancing the result of an agarose gel electrophoresis by marking specific DNA sequences. The method is named after its inventor, the British biologist Edwin Southern.Southern, E.M. (1975): "Detection of specific sequences among DNA fragments separated by gel electrophoresis", J Mol Biol., 98:503-517. PMID 1195397 This caused other blot methods to be named similarly as plays on Southern's name (for example, Western blot, Northern blot, Southwestern blot).