The process of transferring DNA fragments from the electrophoresis gel onto filter paper; named after Dr. E. Southern.
a hybridization technique for detecting the presence of a specific DNA sequence in a genome. The DNA is extracted, cleaved into fragments, separated by gel electrophoresis, denatured, and blotted onto a nitroc ellulose filter. The filter is incubated under annealing conditions with a radiolabeled DNA probe, and the presence of bands of DNA containing the sequence of interest is detected by radioautography.
A method of transfer of bands of DNA, separated on a gel electrophoresis, onto a membrane, from which the bands may be treated with chemicals to enable them to be seen. {Named after the inventor of the process, E.M Southern. Related methods have been named 'Northern Blotting' (RNA blotting from electrophoresis gels) and 'Western Blotting' (protein blotting) by scientists with a sense of humour
Transfer by absorption of DNA fragments separated in electrophoretic gels to membrane filters for detection of specific base sequences by radiolabeled complementary probes.
Procedure for transferring denatured DNA from an agarose gel to a nitrocellulose filter where it can be hybridized with a complementary nucleic acid. ( 13)