A molecule that carries a foreign gene into a host, and allows/facilitates the multiplication of that gene in a host. When sequencing a gene that has been cloned using a cloning vector (rather than by PCR), care should be taken not to include the cloning vector sequence when performing similarity searches. Plasmids, cosmids, phagemids, YACs and PACs are example types of cloning vectors.
Genetic element, usually a bacteriophage or plasmid, that is used to carry a fragment of DNA into a recipient cell for the purpose of gene cloning.
An autonomously replicating genetic element used to carry a cDNA or fragment of genomic DNA into a host cell for the purpose of gene cloning. Commonly used vectors are bacterial plasmids and modified bacteriophage genomes. ( Figures 7-3 and 7-12)
A self-replicating agent that, in the cloning process, serves to transfer and replicate genetic material. Cloning vectors generally are bacterial plasmids or the viruses known as bacteriophages.
Plasmid or bacteriophage element used to transfect a recipient cell with a DNA fragment that is replicated (cloned).
a DNA molecule, such as a plasmid, cosmid, or bacteriophage has the capability of replicating autonomously in a host cell
a nucleic acid molecule, for example, a plasmid, cosmid, or bacteriophage that has the capability of replicating autonomously in a host cell
a plasmid or phage that is used to 'carry' inserted foreign DNA for the purposes of producing more material or a protein product
A DNA molecule capable of autonomous replication within the cloning host cell (e.g E. coli). The vectors contain restriction enzyme sites for insertion of foreign DNA. Cloning vectors are derived from bacterial plasmids, bacteriophages, or viruses.
A piece of DNA, such as a plasmid, into which a DNA segment can be inserted, transferred into an organism, and replicated or reproduced.
A DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment can be integrated without compromising the vector¡¯s capacity for self-replication.
a circular DNA molecule in which foreign DNA is inserted for replication in an appropriate host organism.
A DNA molecule that is capable of replication in a suitable host cell, that has suitable site(s) for the insertion of DNA fragments by recombinant DNA techniques, and that has genetic markers that allow selection for the vector in a host cell.
DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self- replication; vectors introduce foreign DNA into host cells, where it can be reproduced in large quantities. Examples are plasmids, cosmids, and yeast artificial chromosomes; vectors are often recombinant molecules containing DNA sequences from several sources.
any vehicle, such as a virus or a BAC, into which foreign DNA can be inserted for the purpose of replicating or "cloning" large quantities.
Before YACs were developed, the largest cloning vectors (cosmids) carried inserts of only 20 to 40 kb. (IOOakRidge) Vetor de clonagem Desta forma age como um vetor natural de clonagem e vetor de expressão. (POUniverRJ)
A DNA molecule, such as a modified plasmid or virus, that can be used to clone other DNA molecules in a suitable host cell. Cloning vectors must be able to replicate in the host cell and must possess restriction enzyme cut sites that allow the DNA molecules targeted for cloning to be inserted and retrieved.
A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA.
A piece of DNA from any foreign body which is grafted into a host DNA strand that can then self replicate. Vectors are used to introduce foreign DNA into host cells for the purpose of manufacturing large quantities of the new DNA or the protein that the DNA expresses.
A DNA molecule originating from a virus, plasmid, cosmid, phage, bacteria, or yeast into which a foreign DNA fragment is integrated and then introduced into host cells, where it can be reproduced in large quantities (cloned).
An agent used to transfer DNA in genetic engineering, such as a plasmid that moves recombinant DNA from a test tube back into a cell, or a virus that transfers recombinant DNA by infection.
A cloning vector is a small DNA vehicle that carries a foreign DNA fragment. The insertion of the fragment into the cloning vector is carried out by treating the vehicle and the foreign DNA with the same restriction enzyme, then ligating the fragments together. There are many types of cloning vectors.