A method for the dissection of large segments of DNA, in which a cloned segment of DNA, usually eukaryotic, is used to screen recombinant DNA clones from the same genome bank for other clones containing neighboring sequences.
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.
Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest
Using an ordered assembly of clones to extend from a known start point.