in diagnostics, a blood test to measure the concentration of antibodies to a specific antigen; an experimental technique that uses enzymes to detect the presence of specific proteins.
A test used to detect HIV antibodies in a blood sample
(ELISA), is a sensitive, heterogeneous (multiple phase) analytical technique for quantitation of antigen or antibody in which enzyme-labeled antibody or antigen is bound to a solid support (e.g., tubes, beads, microtiter plate wells, plastic tines or fins). After addition of patient specimen and substrate, antigen, antibody or complex are detected by a color change indicating the presence of the product of an enzyme-substrate reaction. Direct ELISA is a technique for measuring antigen using competition for antibody binding sites between enzyme-labeled antigen and patient antigen. Indirect ELISA, or enzyme immunometric assay, measures antibody concentrations using bound antigen to interact with specimen antibodies. Enzyme-labeled reagent antibodies can be isotype-specific (i.e., capable of determining the presence of IgG, IgA, IgM, IgE classes which react with the antigen of interest). The specificity of indirect ELISA assays for IgM isotypes in some infectious diseases is limited by false-positive results due to IgM rheumatoid factor in the presence of IgG-specific antibodies.
blood test used to detect Helicobacter pylori bacteria; also used to diagnose an ulcer.
Enzyme-Linked Immunosorbent Assay (ELISA) is a simple and highly sensitive method of analysis that allows for simultanous and rapid quantification of a large number of samples. The antigen-antibody complex is detected and measured with the aid of an enzyme-labelled antibody or antigen. Upon addition of a non-coloured reagent, the enzyme produces a colour reaction indicating the concentration of analyte in the sample. Türkçe
(acronym ELISA) a serological test in which the sensitivity of the reaction is increased by attaching an enzyme that produces a colored product to one of the reactants
a serological test in which the sensitivity of the antibody-antigen reaction is increased by attaching an enzyme to one of the two reactants.
The ELISA is a fundamental tool of clinical immunology, and is used as an initial screen for HIV detection. Based on the principle of antibody-antibody interaction, this test allows for easy visualization of results and can be completed without the additional concern of radioactive materials use. Ex vivo (testing) The testing of a substance by exposing it to (excised) living cells (but not to the whole, multicelled organism) in order to ascertain the effect of the substance (for example, pharmaceutical) on the biochemistry of the cell.
An assay used to measure the concentration of an antigen or antibody. It is based on the use of an enzyme-antibody conjugate, the enzyme catalyzing a reaction which can be monitored by a color change.
A technique using antibodies for detecting specific proteins. Used to test for the presence of a particular genetically engineered organism.
A laboratory technique for detecting specific antigens or antibodies.
a laboratory test used to detect the presence of antibodies in the serum. ELISA is used for first-line screening for HIV antibodies; a positive result indicates that antibodies have been detected. The test is sensitive but not specific, and a positive ELISA is typically confirmed using a Western Blot assay.
A diagnostic blood test used to screen patients for AIDS or other viruses. The patient's blood is mixed with antigen attached to a plastic tube or bead surface. A sample of the patient's blood serum is added, and excess proteins are removed. A second antibody coupled to an enzyme is added, followed by a chemical that will cause a color reaction that can be measured by a special instrument.
ELISA is now often used for blood meal identification and to determine whether mosquito salivary glands are positive for sporozoites.
Rapid, sensitive and cost-effective test for screening large numbers of serum samples. ELISA kits are commercially available.
This is a blood test used identify certain infections. It is both sensitive and specific. Enzyme Replacement System – This is a chiropractic approach that correlates recurring "subluxation patterns" with the results of a 24-hour urinalysis (purported to identify "enzyme deficiencies") so that spinal adjustments and nutritional measures can be combined. Epi – A Latin prefix meaning above or outside of something. For example, epidural means outside of the dura. Epidermal Nevus Syndrome (Feuerstein-Mims Syndrome) – The combination of epidermal abnormalities combined with central nervous abnormalities.
a sensitive, inexpensive assay technique involving the use of antibodies coupled with indicators (e.g., enzymes linked to dyes) to detect the presence of specific substances, such as enzymes, viruses, or bacteria.
An assay in which an enzyme is linked to an antibody and a coloured substrate is used to measure the activity of bound enzyme and, hence, the amount of bound antibody.
