A laboratory method used to separate molecules. IS 6110-based RFLP uses agarose gel electrophoresis to separate DNA fragments by size.
Electrophoresis carried out in an agarose gel, used to separate DNA molecules whose length is between 100 and 50,000 bp.
a method which is used to separate DNA fragments by molecular weight. Fragments move by electric current at different speeds through the agarose.
A technique for separating DNA molecules, based on the size.
A technique used to separate DNA fragments (and proteins) by their size. An electric current is used to propel the DNA (or proteins) through a porous gel matrix.
A method used to separate, identify and purify molecules of different weight and/or structure. It is specifically applied to the separation of DNA fragments. It is a rapid, simple and accurate process. The separated molecules can be visualized directly by staining with dyes. The electrophoretic migration rate of molecules through agarose gel is dependent on the following parameters: molecular size: molecules pass through the gel at rates that are inversely proportional to the log of their molecular weight. agarose concentration: a molecule of a given size migrates at different rates through gels containing different concentrations of agarose. molecular conformation: a molecule of the same molecular weight but of a different conformation will migrate at different rates. Generally closed circular or globular forms will migrate faster than linear forms. electric current: at low voltages, the rate of migration is proportional to the voltage, but as the voltage is increased the rate of migration of high molecular weight fragments is increased differentially. See Electrophoresis.
Agarose gel electrophoresis is a method used in biochemistry and molecular biology to separate DNA strands by size, and to estimate the size of the separated strands by comparison to known fragments (DNA ladder). This is achieved by pulling negatively charged DNA molecules through an agarose matrix with an electric field. Shorter molecules move faster than longer ones.